Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLE-EAQSIANDLNVTPEQV---LEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRW--LDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 5IPM Chain:F ((61-314)) ----------------------YLGEIGYSPLLTAEEEVYFARRALRG-DVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIV-KELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGG------DSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLF----ELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLR------

General information: TITO was launched using: RESULT: Template: 5IPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 744 -38478 -51.72 -155.15 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain F : 0.77 3D Compatibility (PKB) : -51.72 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_5IPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPM-query.scw PDB file : Tito_Scwrl_5IPM.pdb: