Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRSYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLRKR----GIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 1CUL Chain:C ((114-205)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------VDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV-IKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNF------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 142 -19143 -134.81 -217.53 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain C : 0.53 3D Compatibility (PKB) : -134.81 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.118

(partial model without unconserved sides chains): PDB file : Tito_1CUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CUL-query.scw PDB file : Tito_Scwrl_1CUL.pdb: