Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQGFIKAFANLVGWIFALIMGAKYAVLLAPSMSGLSQDPVVQKIAAFAFIALLIIVLTWIVTAFLNGLLKSLKLGPLNRLAGGAFGSLKGLLVVLITMQGVGPWVESSPHWK--QSKFI---QFLLPYAPLATELSKDAASEAFHQITSGGGVTRTSPKPMDESEETELRPDHSTKDPFY 2J7O Chain:A ((346-431)) ------------------------------------------------------------------------LGLGDVPSAVQGRFGSAKGMWVIDVDDTGDEDWIETYPSQRKWECDFVDKHQRTLEVRSVASEL---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2J7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 139 -6728 -48.40 -114.03 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -48.40 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_2J7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J7O-query.scw PDB file : Tito_Scwrl_2J7O.pdb: