Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMFTLKQFQYFIKIVEEGSFTAASEKLFIAQSALSRQMKLLEEEIDFLLFDRTDKRVKLTAAGEVFYKKIKDNLLYLNEIIDLSKSVSEGKNRQIKIAHSSSIVVDNTKVQILKEVSLTQQLSFEINTLSSEQQILALLNGEIDIGLIR-----PPVRHTLDGINTLKLYEEPLMVAVHIDHA-KFAKSEKVYLKDLKDECFVSTPHAERGGLSYLVANLCLAAGFTPQKASIQSRKIS-QLQLVAANLGVGIVPAEFQQILPANVKLIPLEDSLSLSEVLLVYRK-DHDEIIQHCAEQIHQMFLS 2H99 Chain:A ((90-293)) ---------------------------------------------------------------------------------------------KTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKHSL-------LRNERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKIN-EVREVQLALGLVAAGEGISLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIY--

General information: TITO was launched using: RESULT: Template: 2H99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 -10789 -10.74 -55.05 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -10.74 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2H99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H99-query.scw PDB file : Tito_Scwrl_2H99.pdb: