Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLTSKASLRLTLLASAIFLVACQPKSDPKESEDQQKPAVVEQKPVELTLKGETVPSKVTLPDCDGKTCPEFTVARLQSNFPFIDKIIDQQVLKALGQILEIAEPDAKAAQADKKTEASTAAATEQQDSFDAQVQRYANSFIDLDNDLKALSSNHQINLLVKPKIIQSQGK-VVTVVVNSSSYLGGAHGSAAQQYYNFDLKKEKQVKLEGLLRPEKKAALEKLAHEAFKAWVTDSKLVNSVSEYEQAWPFKL------TENFLLGDQGLILQYGEYEIGPYVVGLPRLVIPYDQLQDVLKEEYLPQPKAKPASTPAVKSTS 3CYG Chain:A ((114-243)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------VRYEGKDFLSFVVYYYQFTGGAHGITFFETYNIDLKNSKVLKLYDIIKEE--------AEDTIKSNILKQIEQNNTDFFPDA-PMNILKDDIFSREFTISKDGLIIMYPHYDLAPYASGMPEFVIPWNVIEKFLKYDIL-----------------

General information: TITO was launched using: RESULT: Template: 3CYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 468 -24966 -53.35 -202.97 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -53.35 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_3CYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CYG-query.scw PDB file : Tito_Scwrl_3CYG.pdb: