Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDYLQALKRIPEARGAWILHGQEPLLEQNLLDTFRKSWQQQDIERQRYDISSVSDWKNVFNALNSLSLF-SQQLAIEVHGNIKPDANGLKQLKSYIQHNETNLLLIVLPKQDSSSLKSAFFQVVEANGVVVALTANYPQDRQ--RILAAEAEKLEIQLDNDAWQWLMQHHEHNLLAAKNSLMRVRDTFPDQKLIQIEQLYACLQDQSRYTTYDLSDALLEGNLAQSIKIFQYLIGSGEPESLILWTLSKEMRLLMQLFEQ----PHNAL--QLGIWKTKVSLYQQALRRLNPQQFLGWSALLLQIDAAIKGMSNENAQHLMQQAIAELCGKTLFIH 1JQJ Chain:C ((49-331)) ---------------------------------------------EHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSV-QVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLT-LPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVW--RRGMMGEALNRLSQTQLRQAVQLLTRTELTLK-------QDYGQ---AELEGLSLLL-

General information: TITO was launched using: RESULT: Template: 1JQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 948 -16878 -17.80 -62.74 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -17.80 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1JQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JQJ-query.scw PDB file : Tito_Scwrl_1JQJ.pdb: