Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRIAINGFGRIGRNVLRAWFESPKQFHFEIVAINDIADVNTLVHLFKYDSTHGRFNGKVDITIENEKIYLNIQSNQRLLKVEVLQQKQPELLPWASLKIDVVLECTGLFRSHADATRHLEAGAKRVIIGAAPFDHVDAAIVYGVNHADVKATDQIISSVSCTTQALVPLVKIIDDAFGIETALMTEIHAVTADQSVLDHAHRDLRRARASGQNIIPTTSSALGALKRVMPKMENRIDGYSIRVPTINVAAIDLTFIAQSPITVHHINELLIKASQTDYAEIMAVTDEPLVSSDFNHSPYSLIVDSTQTMVV-GHQAKVFAWYDNEWGYANRLLDLCDSF 3L0D Chain:A ((25-352)) --RVAINGFGRIGRNILRAIIESGRQ-DIEVVALNDLGSVETNAHLLRYDSVHGCFPGTVQVVGDAIDI---L--------IKVFAERDPAQLPWKALDIDIALECTGIFTARDKASAHLDAGAKRVLV-SAPSEGADLTVVYGVNHQFLSKEHHVISNASCTTNCLAPVAQVLHNTVGIEKGFMTTIHSYTGDQPVLDTMHRDLYRARAAALSMIPTSTGAAKAVGLVLPELKGLLDGVSIRVPTPNVSVVDLTFTAKRSTTIEEINTAIRTAAQGSLKGILDYTDEKLVSCDFNHNPHSAIFHNDQTKVIDGQLCRVLVWYDNEWGFSNRMCDTAVAF

General information: TITO was launched using: RESULT: Template: 3L0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1905 -224258 -117.72 -692.15 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -117.72 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3L0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L0D-query.scw PDB file : Tito_Scwrl_3L0D.pdb: