Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQYMTHRCLIAPPEMADDFFANTVIYLARHDEEGAQGIIINRPAGIQIKELLNDLDIDADNVNP-----HEVLQGGPLRPEAGFVLHTGQPTWHSSIAVGENVCITTSKDILDAIAHNEGVGRYQIALGYASWGKNQLEDEIARGDWLICDADMDLIFNLPYDDRWDAAYKKIGVDRTWLASEIGHA 2GZO Chain:A ((2-187)) ---ESLQNHFLIAMPSLDDTFFERTVIYLCEHDEKGAMGLVINKPLGIEVNSLLEQMDLPTEQVSADLAMGSQVLMGGPVSQDRGFVLHTSQPYWANSTELGSGLMLTTSRDVLTAIGSKRSPDKFLVALGYAGWSKNQLEQELADNSWLTIPADHALLFDINHEDRWQQASRSLGFEAWQLSTQAGHA

General information: TITO was launched using: RESULT: Template: 2GZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 801 -11126 -13.89 -61.47 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -13.89 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_2GZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GZO-query.scw PDB file : Tito_Scwrl_2GZO.pdb: