Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVLGGLSAAIMAVSGSVMAADFVAGKDYTVIANPGKVEVPGKIEVREFFWYGCLHCFKLEPHMQTWLKQIPSDVRFVRTPAAMNKVWEQGARTYYTSEALGVRKRTHLPLFHAIQVNGQQIFDQASAAKFFTRYGVPEQKFNSTYNSFAVTAKVAESNKLAQQYQLTGVPAVVVNGKYVVQ-GEDGKVTQVL---NYLIEKERKAK 2MBT Chain:A ((5-189)) -----------------------DYTAGKEYVELSSPVPVSQPGKIEVVELFWYGCPHCYAFEPTIVPWSEKLPADVHFVRLPALFGGIWNVHGQMFLTLESMGVEHDVHNAVFEAIHKEHKKLATPEEMADFLAGKGVDKEKFLSTYNSFAIKGQMEKAKKLAMAYQVTGVPTMVVNGKYRFDIGSAGGPEETLKLADYLIEKERAA-

General information: TITO was launched using: RESULT: Template: 2MBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 792 -84486 -106.67 -466.77 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -106.67 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_2MBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MBT-query.scw PDB file : Tito_Scwrl_2MBT.pdb: