Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKTIGIFGGGQLGRMMAQAALPLNIQCTFFEANTDCPAGVLGQVFSSQDEQ------GLKQFIESADVFSLEFENTPVADVDVLTQTKTLHPPRIALATAQNRLSEKALFDELAIPVAPYRAVDSLESLKKAVAELGLPIVLKTATGGYDGKGQFVLRSEDQIDTAWAELGPA-KSLVAESFVKFSCEVSIIAVRGQNGEVKTWPLAENHHHNGILSHSIVPAPNSEALQPVAQDYITRLLNHLNYVGVLTLELFVTEQGLCANEMAPRVHNSGHWSIEGAVCSQFENHIRAVAGLPLGSTDVVRPTVMINIIGQHPKTKDVLALNGAHLHLYNKSERAGRKLGHITLMPVDSNELTNLCRQLAKILPEPLALTDDMTI 5JQW Chain:A ((6-362)) ----IGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQF----DVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENV-VLAEKAQQIAKILVKEFAYVGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDT-TSRKTVMLNCIGGMPATKDLAALDRVKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLL-QVKKLI------------

General information: TITO was launched using: RESULT: Template: 5JQW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1971 -13486 -6.84 -38.53 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -6.84 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_5JQW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JQW-query.scw PDB file : Tito_Scwrl_5JQW.pdb: