Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKDTRTRKKVTRTVSEGVAHIHASFNNTIVTITDRQGNALAWATSGGQGFRGSRKSTPFAAQVAAEVAGKAALDYGLKNLDVLVKGPGPGRESAVRALGAVGYKINSITDVTPIPHNGCRPPKKRRV 5ME1 Chain:K ((13-129)) -----------RKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV

General information: TITO was launched using: RESULT: Template: 5ME1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 549 -36722 -66.89 -313.86 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain K : 0.93 3D Compatibility (PKB) : -66.89 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.636

(partial model without unconserved sides chains): PDB file : Tito_5ME1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ME1-query.scw PDB file : Tito_Scwrl_5ME1.pdb: