Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLMINKSMKNIYIMGLLLACSGFAMAETVTQQPTGQP--VRTASNPNAIRIVTRPEIMGLWGMEIPNNKKCVEYYNFRSSNDVVIKSGEEWSYGIYEYQPSDDPKEQLSALVMQIKFDNNKVDCSGQKQDQTGDISQYFVQWKNDHTINFCSTAKGEQCFATLRRMLP 4FFM Chain:A ((214-303)) --LAANLPLKGIMDVEAIFGPKGLRVIEIDARFPSQTPTVVYYSSGINLIELLFRAFTDGVEEI---ENKYCIYEHLMFGENGVLIPVGEQ------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 181 -10051 -55.53 -119.65 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -55.53 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.101

(partial model without unconserved sides chains): PDB file : Tito_4FFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFM-query.scw PDB file : Tito_Scwrl_4FFM.pdb: