Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDIYVEFRGKYKVDGESRDSEHKGWLEVNSWSHNIRQPKSATSSSVGGHTAERVEHSDMVFVKDLDATSPKLWEACSAGYTFDEVQIDFYRANGDKRIKYLQIKLKHVLVSSVTPTVNEEGV--PTEAFGLKYAAVEWTYNQQDINGTAKGAVTKK-WSLSNNTASYAA 3EAA Chain:A ((4-160)) --DTYIKLD---KVDGESTDDKHKKWIEVLGFAWGAGNECTMESGTQGLNTG-KAMMSVLRVTKWMDCASVKLASAAVQGQNFPTLELEICTQAGDK-FAFCIYKFTHVAVSSYQCSGATGGSDRPQETIDFAYKEVTWEYVPQDQNGKAGGKIGPEGWSLITN------

General information: TITO was launched using: RESULT: Template: 3EAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 671 -44402 -66.17 -288.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -66.17 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_3EAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAA-query.scw PDB file : Tito_Scwrl_3EAA.pdb: