Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAKEVAIQLTTHDALGAHAR-----DEIGIHE-------NTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLI-MPSVLITGIA-------ALAILGALSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL 5G4J Chain:A ((156-274)) --------------------------------------------------------------------------------------------------------------------EITTGLTVHEPLGAHVRALRIPDVSGIAEVDVPVLLEQSLADVDAAIASLQAAGHGVSVFLFDPLFSTEGLLQLPSGYIEGVATRVRAAGGLVISDEVQSGFGRTGS-------GMWGY----------QMFNVEP-

General information: TITO was launched using: RESULT: Template: 5G4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 323 -18677 -57.82 -188.65 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -57.82 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_5G4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G4J-query.scw PDB file : Tito_Scwrl_5G4J.pdb: