Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVADKIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMT-LGADFIATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEVRRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYTLGQRGGIGLGGMKGASEGAWFVLHKDVTNNRLVVGQGHDHPLMQSTQLWSESIDWVAGEQNIPAEG-LRCTAKTRYRQPDQACTV-FIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVCLGGGVIHHTNAPTPNFI 2DET Chain:A ((18-379)) --KKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSR------LLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWVDRE---PFTGTMRCTVKTRYRQTDIPCTVKALDDDR---IEVIFDEPVAAVTPGQSAVFYNGEVCLGGGIIEQ-RLPLP---

General information: TITO was launched using: RESULT: Template: 2DET.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2008 -48815 -24.31 -135.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -24.31 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2DET.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DET-query.scw PDB file : Tito_Scwrl_2DET.pdb: