Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEHRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRAQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAHGRPNALDPDEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNTLLEILVPDFRGRMDIALRIMTECPPDVFNHNIETVPRLYKAMRPGSDYQHSLNLLKMFKEYCPDIPTKCGLMVGIGETEEEVIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRKLGFRNIWSAPMVRSSYFADRQYYGEPVPEVRRKVDPAKKIAVQAIEA 5EXJ Chain:A ((51-319)) ----------------------------------------TRARIGPEYTELKNLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFNGE-PTRLAEVFESGPEVLAHNVETVPRIFKRIRPAFTYRRSLGVLTAARD--AGLVTKSNLILGLGETSDEVRTALGDLRDAGCDIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLY-------------------------

General information: TITO was launched using: RESULT: Template: 5EXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1498 -144010 -96.13 -535.35 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -96.13 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_5EXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EXJ-query.scw PDB file : Tito_Scwrl_5EXJ.pdb: