Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDGLGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRFTLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDTIVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGLRGSKASVNKFKPDLFFAVDCSAADDT--VTKNGTFGHLGEGTLMRIQDPGLIMLPRLREYLLDIAETNNIPYQY-FVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFEAAREMLIQTLKGLDKSTVNTIVYGK 1VHE Chain:A ((7-362)) --DETLTMLKDLTDAKGIPGNEREVRQVMKSYIEPFADEVTTDRLGSLIAKKTGAE-NGPKIMIAGHLDEVGFMVTQITDKGFIRFQTVGGWWAQVMLAQRVTIVTKKGEITGVIGSK-PPHILSPEARKKSVEIKDMFIDIGASSREEALEWGVLPGDMIVPHFEFTVMNNEKFLLAKAWDNRIGCAIAIDVLRNLQNTDHPNIVYGVGTVQEEVGLRGAKTAAHTIQPDIAFGVDVGIAGDTPGISEKEAQSKMGKGPQIIVYDASMVSHKGLRDAVVATAEEAGIPYQFDAIAGGGTDSGAIHLTANGVPALSITIATRYIHTHAAMLHRDDYENAVKLITEVIKKLDRKTVDEITY--

General information: TITO was launched using: RESULT: Template: 1VHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2189 -129430 -59.13 -366.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -59.13 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_1VHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHE-query.scw PDB file : Tito_Scwrl_1VHE.pdb: