Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHQVYIDLIMSVLLEQFETEKNFL-------DEYLLISPEQWQKWKDGEKNLPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAE-----------YKQIKTRIAQKWLSSKAAKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWVNENLEETYVHEANGEREEQRKDFSSE 5LTC Chain:B ((223-325)) ----------------YEVQKNLAMTHHIVNDQMVIISESTWQKLSDTDKDIIQKAVQKVGDAHT--QTVKTQEAELVSFFKSEGINVTYPDLEPFREAMQPLYKEFDSNIGQPIVSKLAA-----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5LTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 104 4081 39.24 48.01 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 39.24 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_5LTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LTC-query.scw PDB file : Tito_Scwrl_5LTC.pdb: