Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDNKHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEEFSQEEHLVFICGHYEGYDER-IRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVLGNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQRRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK 1UAK Chain:A ((21-263)) MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAA-GEGAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVL---------SFADGLLDCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQAE----------

General information: TITO was launched using: RESULT: Template: 1UAK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1000 -94270 -94.27 -404.59 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -94.27 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_1UAK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UAK-query.scw PDB file : Tito_Scwrl_1UAK.pdb: