Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQARKRILIGGGSEQPMRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSSTKTAS---TTEQTMATSNNEK----KTALDQLKEQQPNVPM-PL-DVPVSSGYLNIAATHTKQGY-SILYYRTDRP------LGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSRITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFGKITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL 5FQF Chain:A ((75-199)) -----------------------------------------------------TNSSPVNNVTLSSSDFINENGDLISSNNIKISWLKETLANIGRSNPSAPLEPFPDIIHNSGSLNIEKNKIASAWINIKIPRNAKPGIYNGSIEVTADELEK-----SYTFDYSF----EVLNLVQPLPSETNTQ--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 397 42846 107.92 393.08 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 107.92 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_5FQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FQF-query.scw PDB file : Tito_Scwrl_5FQF.pdb: