Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSCYLITGGAGFIGSTLANHLGEE-HVVIVVDDLSMGKVENLDMTKNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLKRIVFSSS-AAVYGDE-PTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEERSGDIHDSLADITKI-QSIGYQPKYGILSGMTNYLKTEIN 4ZRN Chain:A ((7-300)) ----LVTGGAGFIGSHVVDKLIENGYGVIVVDNLSSGKVENLN--RNALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVREPARDAKTNIIGSLVLLEKSIKY--GVKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDPYGE-AGVVAIFTER---MLRGEEVH--IFGDGEYVRDYVYVDDVVRANLLAM--EKGDNEVFNIGTGRGTTVNQLFKLLKEITGYDKEPVYKPPRKGDVRKSILDYTKAKEKLGWEPKVSLEEGL---------

General information: TITO was launched using: RESULT: Template: 4ZRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1655 -28178 -17.03 -97.17 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -17.03 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4ZRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRN-query.scw PDB file : Tito_Scwrl_4ZRN.pdb: