Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMIKDIRFWVCVIGIVILGFLSGLLS-----GNPGKYYYSLQLPPFAPPSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLV--GLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHRL---AMWLMIPYYLWVLFATLLNYSIYFLN 2N02 Chain:A ((5-158)) ---------WVPAVGLTLVPSLGGFMGAYFVRGEGLRWYAGLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADL-LLVSGVATATTLAW--HRVSPPAARLLYPYLAWLAFTTVLNYYVWRDN

General information: TITO was launched using: RESULT: Template: 2N02.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 510 -71433 -140.06 -496.06 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -140.06 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_2N02.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N02-query.scw PDB file : Tito_Scwrl_2N02.pdb: