Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNVAPLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLN-ELKDEKDLLEHAVPI-ITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITY--DLSHPDELFHAITHDKKARGSQLNIILLEKIGQAKIVPVPTETFKDYLGGL 3OKF Chain:A ((33-372)) -ITVNLGERSYPISIGAGLF--ANPALLSLSAKQKVVIVTNHTVAPLYAPAIISLLDHIGCQHALLELPDGEQYKTLETFNTVMSFLLEHNYSRDVVVIALGGGVIGDLVGFAAACYQRGVDFIQIPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPKAVVIDTDCLTTLPAREFAAGMAEVIKYGIIYDSAFFDWLEAQMEALYALDEQALTYAIARCCQIKAEVVAQDEK--GIRALLNLGHTFGHAIEAHMGYGNWLHGEAVSAGTVMAAKTAQLQGLIDASQFERILAILKKAHLPVRTPENMTFADFMQH-MMRDKKVLAGELRLVLPTSIGTSAVV--------------

General information: TITO was launched using: RESULT: Template: 3OKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1771 -147274 -83.16 -440.94 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -83.16 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3OKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OKF-query.scw PDB file : Tito_Scwrl_3OKF.pdb: