Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSIMVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEE-YNELPIIANVAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKVSSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGLSGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPYICPKLIKELPVRMSELGIESLERLIKEVREERER 1EP1 Chain:A ((5-311)) ---NRLSVKLPGLDLKNPIIPASGCFGFGEEYAKYYDLNKLGSIMVKATTLHPRFGNPTPRVAETASGMLNAIGLQNPGLEVIMTEKLPWLNENFPELPIIANVAGSEEADYVAVCAKIGDAANVKAIELNISCPNVKHGGQAFGTDPEVAAALVKACKAVSKVPLYVKLSPNVTDIVPIAKAVEAAGADGLTMINTLMGVRFDLKTRQPILANITGGLSGPAIKPVALKLIHQVAQDVDIPIIGMGGVANAQDVLEMYMAGASAVAVGTANFADPFVCPKIIDKLPELMDQYRIESLESLIQEVKEGKK-

General information: TITO was launched using: RESULT: Template: 1EP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1815 -82285 -45.34 -268.90 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -45.34 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_1EP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP1-query.scw PDB file : Tito_Scwrl_1EP1.pdb: