Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMDVKAYILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGTSAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRPTVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKNK 3J3W Chain:C ((101-277)) -----KRYILAPKGIQVGTEIMSGPEADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGTSAQVLGKEGKYVLVRLNSGEVRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK

General information: TITO was launched using: RESULT: Template: 3J3W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 646 -28464 -44.06 -160.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.95 3D Compatibility (PKB) : -44.06 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_3J3W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J3W-query.scw PDB file : Tito_Scwrl_3J3W.pdb: