Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGRKTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAVYQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQRNENPVKSGTGELTI 4M7V Chain:A ((9-171)) MLAAIWAQDEQGVIGKEGKLPWHLPNDLKFFKEKTIHNTLVLGRATFEGMGCRPLPNRTTIVLTSNPDYQAEGVLVMHSVEEILAYADKYEGVTVIGGGSVVFKELIPACDVLYRTMIHETFEGDTFFPEIDWSVWEKVATVPGVVDEKNLYAHDYETYHRND------------

General information: TITO was launched using: RESULT: Template: 4M7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 808 -113036 -139.90 -693.47 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -139.90 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_4M7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7V-query.scw PDB file : Tito_Scwrl_4M7V.pdb: