Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNLKSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE 1QFR Chain:A ((2-88)) -EKKEFHIVAETGIHARPATLLVQTASKFNSDINLEYKGKSVNLKSIMGVMSLGVGQGSDVTITVDGADEAEGMAAIVETLQKEGLAE

General information: TITO was launched using: RESULT: Template: 1QFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 484 -41564 -85.88 -477.75 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -85.88 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_1QFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QFR-query.scw PDB file : Tito_Scwrl_1QFR.pdb: