Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFDELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIFSGMISSFLILCITALFLSSIISYEKRKQVIS--LVNLLPDSMVKKHSIRFTIYYGAAMLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY 2FWR Chain:A ((197-264)) ----------------------------------------------------------------------------------------------------------------------------------------------IFDEVHHLPAESYVQ-IAQMSIAPFR-LGLTATFEREDGRHEILKEVVGGKVFELFPDSLAGKHLAKYTI---------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FWR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 169 -34427 -203.71 -521.61 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -203.71 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_2FWR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FWR-query.scw PDB file : Tito_Scwrl_2FWR.pdb: