Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK 3N39 Chain:C ((60-147)) ---------------------------------------------------EPYILIVPSYGGGGTA-----GAVPRQVIR-FLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVRKGVTE---

General information: TITO was launched using: RESULT: Template: 3N39.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 324 -49497 -152.77 -562.47 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -152.77 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_3N39.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N39-query.scw PDB file : Tito_Scwrl_3N39.pdb: