Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFDVERMIQMKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKENVS-YVSFDLTWSVEKMTEAFKGIDVLIFAAGSQ----GKNLLQVDLDGAIKTVIAAENAHVSRYLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQLTKPNEKASKTITRSTVAAVLAALVEETD-ISRTTLVLSEGSKELNTAFQEWAKEE 3E8X Chain:A ((23-222)) ------------RVLVVGANGKVARYLLSELKNKGHEPVAMVRNEEQGPELRERGASDIVVANLE---EDFSHAFASIDAVVFAAGSGPHTGADKTILIDLWGAIKTIQEAEKRGIKRFIMVSSVGTVDPDQGPMNMRHYLVAKRLADDELKRSSLDYTIVRPGPLSNEESTGKVTVSPHFSEITRSITRHDVAKVIAELVDQQHTIGKTFEVLN-----------------

General information: TITO was launched using: RESULT: Template: 3E8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -23769 -23.98 -122.52 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -23.98 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_3E8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E8X-query.scw PDB file : Tito_Scwrl_3E8X.pdb: