Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEILIALVPMLAWGSIGLVSGKIGGD--ANQQTLGMTIGAFLFSLVVFFVVS--PVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIAGAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIFSTIGYGVYTIIVNWA-NLDAMSIILPQSIGMILGASFFAFRKV-K-VDQFVW-KNMICGLL-WGLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVILGGILLGYMKTV 5I20 Chain:D ((6-285)) -ATLIGFTAILLWSTLALATS-STGAVPPFLLTALTFTIGGAVGIAAGLARGVG---LRQPWPVWVHGIGGLFGYHFFYFSALKLAPPAEAGLVAY-LWPLLIVLFSAFLPGERLRPAHV----AGALMGLAGTVVLLGA-AGGFG----FAPEYVPGYLAAAACAVIWSVYSVASRRFARVPTEVVAGFCLATAALSALCHILFEPSVWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGAL----AIACALIVGGAAVATLLA--

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1292 -179020 -138.56 -670.49 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : -138.56 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: