Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIVSAEKFVQ---AARDNGYAVGGFNTNNLEWTQAILRAAEAKKAPVLIQTSMGAAKYMGGYKVARNLIANLVESMGIT--------VPVAIHLDH------GHYEDALECIEVG---------YTSIMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAKAMVET------GIDFLAAGIGNIHGPYPVNWEGLDLDHL---QKLTEALPGFP-------IVLHGGSGIPDEQIQAAIKLGVAKVNVNTECQIAFANATRKFARDYEAN-------EAEYDKKKLFDPRKFLADGVKAIQASVEERIDVFGSEGKA 3ELF Chain:A ((2-328)) -PIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGV-KDMVTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIEIGVVGG----------YTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGLPADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAF---TRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYL----KKAEASMSQRV---------

General information: TITO was launched using: RESULT: Template: 3ELF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1448 -10384 -7.17 -39.18 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -7.17 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_3ELF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ELF-query.scw PDB file : Tito_Scwrl_3ELF.pdb: