Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MQNQTASNLPSTQLGKALLCLMTS---ALLFSIMGVCIRFASQTVDNATVVFFRNAVGLFIFIPMLFKQGLDFIKTDKLWMHTWRSIVGLAAMYGFFYAIANLKLSNAMVFSYSSPIFIPLIAWLFLKEKITKSMIFAAVIGLIGVLFVAKPDQGLFNALSFIGLGACFLSAMAFVTVRALTSTEPPERIVFYFCVFGSLISSIPMFWHWRIFTWHELVLLIAAGLLANISQLFMSYAYSLAPAGQIGPMNYIAIIFAGIWGFVFWHELPDLFSIIGIFIILFAILLCNPFLQKKLLSRFK- 2ZCU Chain:A ((1-283)) MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQGITVRQADYGDEAALTSALQG-VEKLLLISPQHRNVINAAKAAGVKFIAYTSLLH-ADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEH------GVFIGAAGDGKI---------ASATRADYAAAAARVISEAGHEGK---VYELAGD-----------SAWTLTQLAAELTKQSGKQVTYQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSK------TLSKLIGHPTTTLAESVSHLFN

General information: TITO was launched using: RESULT: Template: 2ZCU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1452 -76395 -52.61 -292.70 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -52.61 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_2ZCU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZCU-query.scw PDB file : Tito_Scwrl_2ZCU.pdb: