Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRTFPCPRCGEPSVWEGNEFRPFCSERCKLIDLGAWANDEYRLPTQDAPQQDKGSQHEDDYED 3WWL Chain:A ((1-15)) --MVGTCPECGAELRLE-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 20 -886 -44.30 -59.07 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -44.30 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.914

(partial model without unconserved sides chains): PDB file : Tito_3WWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WWL-query.scw PDB file : Tito_Scwrl_3WWL.pdb: