Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRIPVINTSHLDRIDELLVDNIDTGEFKLHRSVFTDEALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPIIIARNRNGELNAMMNACSHRGAQLCRYKRGNKATYTCPFHGWTFNNSGKLLKVKDPTDAGYSDCFNQDGSHDLKKV------ARFESYKGFLFGSLNPDVPSLEEFLGETTKIIDMIVDQSEHGLEVLRGSSTYTYEGNWKLTAEN-GADGYHVSAVHWNYAATTQHRKETQAADNIRAMSAGSW--GKQGGGSYGFENGHMLLWTQWANPEDRPNF---PKADEYTEKYGEAM-SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPLSVNRTEVTIYCI----AP---KGEARARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIALEWNDMCRGSKHWIYGPDDAANEIGLKPMLSGIKTEDEGLYLAQHQYWLKTMKHAIATEKEIADQGETA 1WQL Chain:A ((22-410)) ---------------DEEIKALVDEEKGLLDPRIFSDQDLYEIELERVFARSWLLLGHEGHIPKAGDYLTTYMGEDPVIVVRQKDRSIKVFLNQCRHRGMRIERSDFGNAKSFTCTYHGWAYDTAGNLVNVPYEKEAF---CD-----CGFDKADWGPLQARVDTYKGLIFANWDTEAPDLKTYLSDATPYMDVMLDRTEAVTQVITGMQKTVIPCNWKFAAEQFCSDMYHAGTMA-HLSGVLSSLPPEMDLSQVKLPSSGNQFRAKWGGHGTGWFNDDFALLQAIMGPKVVDYWTKGPAAERAKERLGKVLPADRMV--AQHMTIFPTCSFLPGINT-VRTWHPRGPNEIEVWSFIVVDADAPEDIKEEYRRKNIFT----FNQGGTYEQDDGENWVEVQRGLRGYKARSRPLC--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1WQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1883 -29870 -15.86 -82.06 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -15.86 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1WQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WQL-query.scw PDB file : Tito_Scwrl_1WQL.pdb: