Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEARVIGLEKLGKHDVE-LVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGLNARIQAELAELNVDDVNALAETGAKIRQWIVETPLTPGLEQEIREAF--------AALSNGNPDIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIA-------------------------VKEVFASLFNDRAIAYRVHQGFEHSLVALSAGVQRMVRSETG----AAGVMFTLDTE-------------------SGFRDVVFITASYGLGEMVVQGAVNPDEFYLSKPLLNAGKHAVLRRNLGSKHQKMIYGEE--GSAGKSVVV-VDVEKQERQQFALNDHELQELAKQALIIEKHYGAPMDIEWAKDGDDGQIYIVQARPET------------------VKSRQNVGTMERYLLKQ------------KGTVLCEGRSIGQRIGSGKVRIVNSIKEMDKVQ-DGDVLVSDMTDPDWEPVMKRAAAIITNRGGRTCHAAIITRELGVPAIVGCGNA-----TEVLTDGQEVTVS----CAEGDTGFIYEG---------ALDFEVQRNSIESMPKLSFKIMMNVGNPDRAFDFAQIPNEGIGLARLE---FIINRMIGVHPKALLNIESLPRETRAAVMTRTAGYASPVEFYVEKLVEGIATLAAAFADKPVIVRMSDFKSNEY--------------------ANLIGGKLYEPEEENPMLGFRGASRYISDNFRDCFELECRALKKVRDEM---GLTNI-QIMIPFVRTVSEAKR---VIELLAQNGLKRGENGL----KVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRD-----------SGIVSH----LFDERDAAVKALLSMAIHACRKAGKY--VGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAEEKIKQI 5JVL Chain:A ((14-859)) -----------GNRDMKSLLGGKGANLAEM----SSIGLSVPPGLTISTEACEEY-QQNGKS---------------------------------LPPGLWDEISEGLDYVQKEMSASLGDPSKPLLLSVRSGAAI------SMPGMMDTVLNL-GLNDEVVAGLAGKSGARFAYDSYRRFLDMFGNVVMGIPHSLFDEKLEQMKAEKGIHLDTDLTAADLKDLVEKYKNVYVEAKGEKFPTDPKKQLELAVNAVFDSWDSPRANKYRSINQITGLKGT-------AVNIQSMVFGNMGNTSGTGVLFTRN-PSTGEKKLYGEFLINAQGEDVVAGIRTPEDLGTMETCMPEAYKELVENCEILERHYKDMMDIEFTVQ--ENRLWMLQCRTGKRTGKGAVRIAVDMVNEGLIDTRTAIKRVETQHLDQLLHPQFEDPSAYKSHVVATGLPASPGAAVGQVCFSAEDAETWHAQGKSAILVRTETSPEDVGGMHAAAGILTARGGMTSHAAVVARGWGKCCVSGCADIRVNDDMKIFTIGDRVIKEGDWLSLNGTTGEVILGKQLLAPPAMSNDLEIFMSWADQARRL--KVMANADTPNDALTARNNGAQGIGLCRTEHMFFASDERIKAVRKMIMAVTPEQRKVALDLLLP----------YQRSDFEGIFR---AMDGLPVTIRLLDPPLHEFLPEGDLEHIVNELAVDTGMSADEIYSKIENLSEVNPMLGFRGCRLGIS--YPELTEMQVRAIFQAAVSMTNQGVTVIPEIMVPLVGTPQELRHQISVIRGVAANVF--AEMGVTLEYKVGTMIEIPRAALIAEEIGKEADFFSFGTNDLTQMTFGYSRDDVGKFLQIYLAQGILQHDPFEVIDQK--GVGQLIKMATEKGRAANPSLKVGICGEHGGE-PSSVAFFDGVGLDYVSCSP------------------

General information: TITO was launched using: RESULT: Template: 5JVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3558 67229 18.90 98.00 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 18.90 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_5JVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JVL-query.scw PDB file : Tito_Scwrl_5JVL.pdb: