Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAEEHALTSPEYIKHHLTNMTYGKMPDGTWKLAETAEEAHSMGFTAIHLDSMGWSIGLGVIFCLLFWIVARAANAGVPTKFQSAIEMIIEFVDSSVRDTFHGKSRLIAPLALTIFVWIFLMNLMDLIPVDWIPQVAAFVGANAFGMDPHHVYFKIVPSTDPNITLGMSLSVFVLILFYSIREKGVGGFVGELALNPFNPSNPVAKALLIPVNLILELVTFLARPISLALRLFGNMYAGELIFILIALLPFW----------IQWALSVPWAIFHILVITLQAFIFMMLTIVYLSMASEKH 5FIK Chain:W ((32-225)) ---------------------------------------------------------------------PTSNRL--VSNRFVTLQQWMLQLVSKQMMSIHNSKGQTWTLMLMSLILFIGSTNLLGLLPHSF------------------------TPTTQLSMNLGMAIPLWAGAVITGFRNKT-KASLAHFLP--QGT-----PTPLIPMLVIIETISLFIQPMALAVRLTANITAGHLLIHLIGGATLALMSISTTTALITFTILILLTILEFAVAMIQAYVFTLLVSLYLHDN----

General information: TITO was launched using: RESULT: Template: 5FIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain W - contact count / total energy / energy per contact / energy per residue : 663 -106330 -160.38 -577.88 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain W : 0.66 3D Compatibility (PKB) : -160.38 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_5FIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIK-query.scw PDB file : Tito_Scwrl_5FIK.pdb: