Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLWLFEQLAGYHSSF---------QVVRYLTLRSLLS---------VLTSLTI-GLVLGPIMIRKLQALKYGQAVSSFAPENHAKKMGTPTMGGILILLSIGISTLLWADLSNPYVWIVLGVMVVFGAVGWADDWIKIRYKDNAGLPARKKFFWTSVASLGAGI---ALYLIATQQSNAEYTAN-----MLDLLIPFFKNLSIPLSIVPLGLAFIVFTYLVINGASNAVNLTDGLDGLAIMPVVMVATGLGVFAYLSGDIRFANYLHIPYVKYTSELVVICSAMIGAGLAFLWYNAHPAQVFMGDVGALALGAMLGTIAVMVRQEIVFAIMGGVFVMEAVSVFLQIGSLRMRNKRVFLMAPLHHHYEKQGWKETQVVIRFWIITIMLVVLGLMTLKLR-- 2W0C Chain:L ((1-335)) MIVKKKLAAGEFAETFKNGNNITIIKAVGELVLRAYGADGGEGLRTIVRQGVSIKGMNYTSVMLHTEYAQEIEYWVGDLDYSFQEQTTKSRDVNSFQIPLRDGVRELLPEDASRNRASIKSPVDIWIGGENMT--ALNGIVDGGRKFEAGQEFQINTFGSVNYWVSDEEIRVFKEYSARAKYAQNEGRTALEANNVPFF-DIDVPPELDGVPFSLKARVRHKSKGVDGLGDYTS----ISVKPAFYITEGDETTD--------------TLIKYTS---------------YGSTGSHSGYDFDD-----------NTLDVMVTLS------AGVHRVFPVETELDYDAVQEV----------QHDWYDESF---TTFIEVYSDDPLLTVKGYAQILMERT

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1264 -54076 -42.78 -176.72 target 2D structure prediction score : 0.15 Monomeric hydrophicity matching model chain L : 0.64 3D Compatibility (PKB) : -42.78 2D Compatibility (Sec. Struct. Predict.) : 0.15 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.073

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: