Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMSPSSSGHVNMMKGQPFHVKYREIIRRMSFLIGALLVFRLGAHIPVPGINNAALENLFHANQGTILGLFNMFSGGALERMSILALGIMPYISASIIVQLMSTVIPSLEALKKEGEQGKRKINQYTRQGTLLLAVVQAVGMCAGLIGQGI------TLSSGLAFYIPAVTSLVAGTMFLMWLGEQITERGIGNGISMIIFAGIVAGLPKLIMQSVSSVDNGQTSLIGLVIFGLLSLGVLAAIVFIEKAQRRIPVNYAQKQQGRRIFTAQQTHLPLKINMAGVIPAIFASSLLLFPASLGQWVGSADPNAGLIKRSLQDLALVLSPGQPLYLVLFGALIIFFCYFYTALVFSPKEVSENLKRSGAYVPGIRPGEQTARYLDHILNRLTFIGAIYIAVICLMPMILQSSFGIP-FYLGGTSLLIVVVVVMDFMAQLQAHLTFHQYDNQTLMRKTTAHPKG 2ZQP Chain:Y ((2-422)) -------------VKAFWSALQIPELRQRVLFTLLVLAAYRLGAFIPTPGVDLDKIQEFLRTAQGGVFGIINLFSGGNFERFSIFALGIMPYITAAIIMQILVTVVPALEKLSKEGEEGRRIINQYTRIGGIALGAFQGFFLATAFLGAEGGRFLLPGWSPGPFFWFVVVVTQVAGIALLLWMAERITEYGIGNGTSLIIFAGIVVEWLPQILRTIGLIRTGEVNLVAFLFFLAFIVLAFAGMAAVQQAERRIPVQYAR-------YGGQATYIPIKLNAAGVIPIIFAAAILQIPIFLAAPFQDNP--------VLQGIANFFNPTRPSGLFIEVLLVILFTYVYTAVQFDPKRIAESLREYGGFIPGIRPGEPTVKFLEHIVSRLTLWGALFLGLVTLLPQIIQNLTGIHSIAFSGIGLLIVVGVALDTLRQVESQLMLR-----------------

General information: TITO was launched using: RESULT: Template: 2ZQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 1717 -256452 -149.36 -630.10 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain Y : 0.83 3D Compatibility (PKB) : -149.36 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_2ZQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZQP-query.scw PDB file : Tito_Scwrl_2ZQP.pdb: