Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSYRQQLQAKIDRITTQFSEFTPPTLEVFESPEQHFRMRAEFRIWHTENDMFYAMFERNDDGKQKTVVRIDEFPIADKSINDLMPLLLAELKANSLLSQRLFEVDFLATLSGEMLVTLIYHRKLNQEWEQAAKALAEKLNIKIMGRSRGQKIVIGDDFVVEEFELLNRSFKYKQIESS--FTQPNAQVCKKMLQWACDAAEGSKKHLLELYCGNGNFTLPLSLK--FERVLATELAKSSVYAAQWNIEQNQIDNIQVARLSAEEFTQAYQGEREFRRLQEADIDIQSYDFGTVFVDPPRA--GIDDETLKLLQGFERIIYISCNPDT---L---YENLKTLT--QTHRVTKFAL---FDQFPYTHHVESGVLLEKI 3A27 Chain:A ((90-272)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCLFKLDVAKIMWSQGNIEERKRMAFIS----N-ENEVVVDMFAGIGYFTIPLAKYSKPKLVYAIEKNPTAYHYLCENIKLNKLNNVIPILADNRDVEL--K-----------------DVADRVIMGYVHKTHKFLDKTFEFL-KDRGVIHYHETVAEKIMYERPIERLKFYAEKNGYKLIDYEVRKIKKYAPGVWHVVVDAKFERI

General information: TITO was launched using: RESULT: Template: 3A27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 808 8037 9.95 48.41 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 9.95 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_3A27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A27-query.scw PDB file : Tito_Scwrl_3A27.pdb: