Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSLSLLHPYPFEKLNQLFKDTTPANLPLIPLSIGEPKHPAPEFVKQAIIDNFNHLST-YPNSKGLPELRQSIADWLTKRFKLNSISAENHILPVSGTREGIFSFVQTLINREDAPYVVMPNPFYQIYEGAALLAGAKPYFINCTEENGYLGDFDAVPAEVWEKTALLFVCTPGNPTGTVLSKEQFKKLIALSDQYNFVIASDECYSELWFDQAPTGLLEVCAELGRGDYKNCIVFHSLSKRSNLPGMRSGFIAGDAALLKPYLQYRTYHGAAMPVQHQLASIAAW-NDESHVEE------NRKQYRAKFDLFQSELGHLLPLQKPDAGFYYWLKV----DNDETFAKMLMEKAHIKVLPGRYLSRDTEQGNPGANHVRMALVADLAQCEEVVKRLKAIL 1J32 Chain:A ((29-384)) --------------------------GIDVCSFSAGEPDFNTPKHIVEAAKAALEQGKTRYGPAAGEPRLREAIAQKLQRDNGL--CYGADNILVTNGGKQSIFNLMLAMIEPGDE--VIIPAPFWVSYPEMVKLAEGTPVILPTTVETQFKVSPEQIRQAITPKTKLLVFNTPSNPTGMVYTPDEVRAIAQVAVEAGLWVLSDEIYEKILYDDAQH--LSIGAASPEA-YERSVVCSGFAKTYAMTGWRVGFLAGPVPLVKAATKIQGHSTSNVCTFAQYGAIAAYENSQDCVQEMLAAFAERRRYM--LDALNAMPGLECP--KPDGAFYMFPSIAKTGRSSLDFCSELLDQHQVATVPGAAFGAD--------DCIRLSYATDLDTIKRGMERLEKFL

General information: TITO was launched using: RESULT: Template: 1J32.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2013 -49951 -24.81 -145.20 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -24.81 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_1J32.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J32-query.scw PDB file : Tito_Scwrl_1J32.pdb: