Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MENFEVIKALFLGFVEGLTEFLP-----------------------------ISSTGHLILFGHIIDFHSDGGRVFEVVIQLGAILAVCWLYRQKIINLIKG--FFSGDVESRHFAISVLIAFF-----------------PAVIIGVLAVDFIKSVLFSP----IVVAIALIVGALIIFWVESK-----QFEHKTDDATKITFKQALLVGLAQ-----------CVAMIPGTSRSGATIVGGMFAGLSRK-AATEFSFFLAMPTMLGAA--------TFDLIKNADVLTSDNMVNIGVGFVAAFIAALLVVKALV----------LFVERHTLRVFAWYRIVLG-------VIILIAAMFFNLSA---------- 5LEV Chain:A ((9-404)) PMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIILFCFIPFPFLPHHEFVALI--GALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAGVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVR

General information: TITO was launched using: RESULT: Template: 5LEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1300 -229550 -176.58 -850.18 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -176.58 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_5LEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LEV-query.scw PDB file : Tito_Scwrl_5LEV.pdb: