Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQYQPPHINRKLNVQAWWLTALLISINVGLFIWQIISGVDISDPAIKDALHWGADFTPLTFSGQPERLFTSMFFHFGIIHLLLNMWALYIFGNVAEALFGRLYFIGLYLLAGLFGSLLSSYINIQNGHELLQHFDQSLLPHVSAGASGAVMGLGAALTVLSLFPPLPHQAYILDKKALLMIMAINLI---FGFVATGINNAAHVGGMIMGALLALIWYLSYRTPFKSLLKFLGLVGGLFMTVGFYVYCNQLNSPLLPLWHEVLIQNPEILP 3ZEB Chain:A ((36-176)) --------------------------------------------------------FDP-TLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGS----GKLIVITLISALLSGYVQ--------QKFSGPWF----GGLSGVVYALMGYVWLRGERDP---QSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFV-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZEB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 543 -95940 -176.69 -695.22 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -176.69 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_3ZEB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZEB-query.scw PDB file : Tito_Scwrl_3ZEB.pdb: