Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAYDADLELFRDNFKRFMNEHIAPHYDQWEREGIMPRSVWSQLGENGFLCVDVPEEYGGYGVPTYYSLMLVEESARAGFCALSTAISCHSEIAAPYILHIGTEEQKQYWLPKMVTGEVVGAIGMTEPGAGSDLQSMRTSAILQDDHYLLNGSKTFISNGQHADLVVLAVKTDPQARAKGVSLLLVDTYLEGFKKGTNLDKIGLHSQDTSELFFDNVKVPKNQLLGQAGQGFAYLMQELPRERTAIASTAVGAIRGAIDLATAYVKERYAFGQPISQFQNTRFVLAQAKIDELATTAFYERNVALYQEGKLDVETAAALKSFSTDMQMKVADNLLQLFGGYGYMTEYPISRFFVDARIQRIYGGTNEIMKEIVARGLIGKA 2VIG Chain:A ((11-379)) --------QMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISR-GCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKSISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLL---

General information: TITO was launched using: RESULT: Template: 2VIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2194 -187315 -85.38 -507.63 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -85.38 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_2VIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VIG-query.scw PDB file : Tito_Scwrl_2VIG.pdb: