Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQNSQKWLKTVIEKKEKALDIQGLFTNFRFPRKFSVFIF 2PQ4 Chain:B ((1-35)) MKLSRRSFMKA--NAVAAAAAAAGLSVPGVARAVVGQ---

General information: TITO was launched using: RESULT: Template: 2PQ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 45 89 1.98 2.54 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.51 3D Compatibility (PKB) : 1.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_2PQ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PQ4-query.scw PDB file : Tito_Scwrl_2PQ4.pdb: