Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVITIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLEILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLSHPLSIHVRNEWQLADSQLLFQEK 1EN5 Chain:A ((19-83)) ------------------------------------------------------------------------------------------------DKQTMEIHHTKHHQTFVNNANAALESLPEFANLPVEELITKLDQLPADKK--TVLRNNAGGHANHSL--------------------

General information: TITO was launched using: RESULT: Template: 1EN5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 179 -9980 -55.75 -153.54 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -55.75 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_1EN5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EN5-query.scw PDB file : Tito_Scwrl_1EN5.pdb: