Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRVRLRVPKQWDRFQVNIDEELQQEELSRTLTEREIDDFNRRNLHSVSELSDHLRLLVFGSDECVTCSEDICAAVEYIVGPQRRWNSSCDEVCEFRRAISRAYLNEWKAFQSVHPSVPERLATRSLPQVFSVPPLQKAQMDLLMQMCPRNAETGDGSISMRLWYLDSAGSRCSTRRRFPFDTAADDAIRLTMSSAREFASMREKTDDTPLIFVFRVKGRNEYIYGSQPLINYRYIRHCVSKRAEIDLVVEPKEATLFAVPL---FRPSYPSRTLASPSDADASKSSTSEICLWDLTSKLSIRIVGGFEKLAISASRMKDENVRDSSSLYFAVLVEAYVGTWRCCEPQITDWQLCENAKNSHSLAARVYWKDGGTVTFSIDLSNVPRELKLCFTLVASSTDRIVGARAATAEEVIAAVEVNTKKTDDQGHSVFFLGTAATQLFDYAAKMRMGMWRIYLWEG----KTRANPIGLNSINPDSNACTFEVEFPCFDKPVFFPSGRPPKRKEEDA------RRNFADREAALSSYLI-NNEVEQ--LRQLKRVLMYDPLVHLSSDDKTILWKYREMLLGQPKAMAKVMSAVNWLMPFDVYEAHCLLRRWSPLAAFDALELLDAHYADLAVREQAVEYIDK-MSDYELRGCILQLVQVLKYEPYHYSALARFLLRRSLRSNHIIGHYVFWYLAAEVKNLTICERHGLLLEELIKRSPHRLDYV-RQVYVCNELLKCALRVQRAPKKDRVKCLRAS--LAKVRFP------HRFTLALNPSVECCAVDIDECKVMESKKFPLWLAFRNHLDQDEHFF----IIFKSGDDLRQDLLTLQLLEFMDSLWKASGLDLHLIPYGCISTGEGVGMIEVVLNSDTIANITRREGGAQAAFNVDPIINWLRQFNHDRGEV-ERCLWNFVLSVAGYTVATYVLGIGDRHNDNIMLRQDGTLFHIDFGHFLGNFKTKFGIKRETAPFIFTPMYLYAMGGSSSPI-----FKYFVDVACRAYNALRRHSSALMMLFMLMLSTGIPELQTLEDIEWLRTVLLLNRTDEEASDHYKGLINDALGNFRTLLNDYIHIMVH 4L23 Chain:A ((250-1058)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYS-----------GETSTKS---LWVINSALRIKIL---------CATYVNVNIRDIDKIY------VRTGIYHGGEP------LCDNVNTQRVPCSNPRWNEW--LNYDIYIPDLPRAARLCLSICS------VKG---AKEEHCP------------------LAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDM---SVIEEHANWSVSREA----------NRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKAL-TNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLT-DILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCK--------NSHTLHQWLKDKN--KGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWI-----

General information: TITO was launched using: RESULT: Template: 4L23.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3638 -122265 -33.61 -165.22 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -33.61 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4L23.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L23-query.scw PDB file : Tito_Scwrl_4L23.pdb: