Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------RPYHGRIQDLRRPLLCDVYIEYPSGQVFDQALGHDIVPAGETAEISGFKCKTVRGTCVILPTCDTPPEYRVTSWKFEREM----SDPERTRFIARFPNVRI-------------------- 3WIS Chain:A ((19-198)) FKPDARFAWCVTGSGHLLDESIALALELPRADLFLSAAAEEVLPLYGWALPRLRKHFRVFRDNSASGVPVGMLYHGMYHTVVIAPAT---SNTVAKCAFGISDTLPTNMYAQAGKQCIPGIVFACDTEPTVVTQSPNEWVELRPRAIELDNVERLSRFEYTTLVRSLDELKAALGERLSTLDL

General information: TITO was launched using: RESULT: Template: 3WIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 381 -8751 -22.97 -93.09 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -22.97 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.118

(partial model without unconserved sides chains): PDB file : Tito_3WIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WIS-query.scw PDB file : Tito_Scwrl_3WIS.pdb: