Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----GCQVELLNINQAVVGSGCVPFNYYANIYDSITRAGYTVEANINCGLKFQAGRRIPDGYSLRRAGYC------------------------------------- 2VKJ Chain:A ((1-106)) GSHMNLAVKLTRMEKTLKAYELYIFSDYENFENYVKKEGLKIE-GMELLKEKKARSLIAEGKDLFETANYGEALVFFEKALNLSDNEEIKKIASFYLEECRKKLAGD

General information: TITO was launched using: RESULT: Template: 2VKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 106 3175 29.95 48.84 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 29.95 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_2VKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VKJ-query.scw PDB file : Tito_Scwrl_2VKJ.pdb: