Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIKQYLT----ATERVSIIHTDEKIDSDD-------------EPTRAIRNKKNASMVLAAKAVKDGEADAVLSAG--NTGALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSFYAKNVRGIAQPRVGLLNNGTESSKGDP---LRKETYELLAADESL-NFIGNVEAR--------------DLMNGVADVVVADGF-TGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLNYSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE 1XCO Chain:D ((31-328)) -------------LDERILEAVSKLAGNK-VLNPIVIGNENEIQAKAKELNLTLGGVKIYDPHTYEGMEDLVQAFVERRKGKATEEQARKA-LLDENYFGTMLVYKGLADGLVSGAAHSTADTVRPALQIIKTKEGVKKTSGVFIMARG-EEQYVFADCAINIAPDSQDLAEIAIESANTAKM-FDI-EPRVAMLSFSTKGSAKSDETEKVADAVKIAKEKAPELTLDGEFQFDAAFVPSVAEKKAPDSEIKGDANVFVFPSLEAGNIGYKIAQ------------RLG-------------------------NFEAVGPILQGLNMPVNDLSRGCNAEDVYNLALITAAQA---------------

General information: TITO was launched using: RESULT: Template: 1XCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1251 25837 20.65 99.37 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : 20.65 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1XCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XCO-query.scw PDB file : Tito_Scwrl_1XCO.pdb: